{"id":4190,"date":"2025-08-02T13:00:14","date_gmt":"2025-08-02T11:00:14","guid":{"rendered":"https:\/\/www.fzmb.de\/research\/detect-bee-infections-early\/tests-for-american-foulbrood\/"},"modified":"2026-02-06T10:51:15","modified_gmt":"2026-02-06T09:51:15","slug":"tests-for-american-foulbrood","status":"publish","type":"page","link":"https:\/\/www.fzmb.de\/en\/research\/detect-bee-infections-early\/tests-for-american-foulbrood\/","title":{"rendered":"AFB duplex rapid test American foulbrood"},"content":{"rendered":"\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 38%\"><div class=\"wp-block-media-text__content\">\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-bienenseuche-amerikanische-faulbrut\"><strong>American foulbrood bee disease<\/strong><\/h2>\n\n\n\n<p>American foulbrood (AFB) is a highly infectious disease of bee larvae that leads to death. A quick and simple test of dead larvae on site provides immediate clarity as to whether an infection with American foulbrood is present. This offers the opportunity to initiate rapid countermeasures in the event of an AFB infection and prevent the spread of the infection. <\/p>\n\n\n\n<p>The rapid tests currently available on the market only detect one of the two dangerous pathogen forms, the <a href=\"#h-eric-i-und-eric-ii-sind-verschiedene-erregertypen-der-afb\">ERIC I genotype<\/a>, but not ERIC II. <em>P. larvae<\/em> ERIC I infections lead to the typical foulbrood symptoms, in which the brood &#8220;rots&#8221; into a stringy mass after capping. However, honey bees are also infected with the pathogen <em>P. larvae<\/em> genotype ERIC II with the same frequency. This kills the larvae before they hatch. For this reason, the bee disease AFB is often not recognized in cases of infestation by the ERIC II genotype. This in turn favors the spread of the disease. American foulbrood infections are transmitted from one bee colony to the next, endangering entire colonies.<\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"907\" height=\"1024\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut-907x1024.jpg\" alt=\"\" class=\"wp-image-4695 size-full\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut-907x1024.jpg 907w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut-266x300.jpg 266w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut-768x867.jpg 768w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut-1361x1536.jpg 1361w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Titelfoto-AFB-Duplex-Schnelltest-mit-Frueherkennung-auf-Amerikanische-Faulbrut.jpg 1552w\" sizes=\"auto, (max-width: 907px) 100vw, 907px\" \/><\/figure><\/div>\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 38%\"><div class=\"wp-block-media-text__content\">\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-neuer-afb-duplex-schnelltest-nachweis-bringt-sicherheit\"><strong>New AFB duplex rapid test: Detection provides certainty<\/strong><\/h2>\n\n\n\n<p>We have developed a new detection method for American foulbrood in collaboration with the State Institute of Apiculture. The AFB duplex rapid test quickly and reliably detects whether bee larvae have been infected by the American foulbrood pathogen. In addition, the AFB duplex rapid test makes it possible to differentiate which genotype of the American foulbrood pathogen is responsible for the infection of the bee larvae. Unlike other tests, this test also detects symptom-atypical infections.<\/p>\n\n\n\n<p>This allows control measures against malignant foulbrood to be initiated quickly and prevent the infection from spreading to neighboring bee colonies.<\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"687\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-1024x687.png\" alt=\"\" class=\"wp-image-4526 size-full\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-1024x687.png 1024w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-300x201.png 300w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-768x516.png 768w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-1536x1031.png 1536w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-17-um-19.48.40-2048x1375.png 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 38%\"><div class=\"wp-block-media-text__content\">\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-vorteile-des-afb-duplex-schnelltest\"><strong>Advantages of the AFB duplex rapid test<\/strong> <\/h2>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>On-site detection on the bee colony<\/li>\n\n\n\n<li>Differentiation of AFB infection<\/li>\n\n\n\n<li>Quick and easy to implement  <\/li>\n\n\n\n<li>Early detection of American foulbrood<\/li>\n<\/ul>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"540\" height=\"592\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Bildschirmfoto-2025-09-05-um-14.50.19.png\" alt=\"Illustration Duplex rapid test American foulbrood fzmb GmbH\" class=\"wp-image-4691 size-full\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Bildschirmfoto-2025-09-05-um-14.50.19.png 540w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Bildschirmfoto-2025-09-05-um-14.50.19-274x300.png 274w\" sizes=\"auto, (max-width: 540px) 100vw, 540px\" \/><\/figure><\/div>\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<div class=\"wp-block-advgb-accordions advgb-accordions-06a378dd-223c-4a98-9fe5-2691c959a8bb advgb-accordion-wrapper\" data-collapsed=\"true\">\n<div class=\"wp-block-advgb-accordion-item advgb-accordion-item\" style=\"margin-bottom:15px\"><div class=\"advgb-accordion-header\" style=\"background-color:#F2F2F2;color:#006CC2;border-style:solid;border-width:0px;border-color:#F2F2F2;border-radius:2px\"><span class=\"advgb-accordion-header-icon accordion-state\"><svg class=\"advgb-icon-closed\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M16.59,8.59L12,13.17L7.41,8.59L6,10l6,6l6-6L16.59,8.59z\"><\/path><\/svg><svg class=\"advgb-icon-opened\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M7.41,15.41L12,10.83l4.59,4.58L18,14l-6-6l-6,6L7.41,15.41z\"><\/path><\/svg><\/span><h3 class=\"advgb-accordion-header-title\" style=\"color:inherit\"><strong>Overview<\/strong> The AFB duplex rapid test in use<\/h3><\/div><div class=\"advgb-accordion-body\" style=\"border-style:solid !important;border-width:0px !important;border-color:#F2F2F2 !important;border-top:none !important;border-radius:2px !important\">\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-medium-font-size\" id=\"h-einfache-testung-von-bienenlarven\"><strong>Simple testing of bee larvae<\/strong><\/h3>\n\n\n\n<p>The AFB duplex rapid test (lateral flow assay) works like the well-known corona rapid tests. A suspicious, dead bee larva is required as a test sample. If this has died due to an infection with American foulbrood, the rapid test allows detection within 15 minutes (see illustration).  <\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-medium-font-size\" id=\"h-der-afb-duplex-schnelltest-in-der-anwendung\"><strong>The AFB duplex rapid test in use<\/strong><\/h3>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1302\" height=\"860\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-29-um-10.12.36.png\" alt=\"\" class=\"wp-image-4616\" style=\"width:847px;height:auto\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-29-um-10.12.36.png 1302w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-29-um-10.12.36-300x198.png 300w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-29-um-10.12.36-1024x676.png 1024w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/08\/Bildschirmfoto-2025-08-29-um-10.12.36-768x507.png 768w\" sizes=\"auto, (max-width: 1302px) 100vw, 1302px\" \/><\/figure>\n\n\n\n<div style=\"height:60px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-medium-font-size\" id=\"h-warum-mussen-larven-getestet-werden-u-keine-abstrichproben\"><strong>Why do larvae have to be tested (and not swab samples)?<\/strong><\/h3>\n\n\n\n<p>Unfortunately, it is not possible to detect the pathogen in American foulbrood from swab samples (e.g. from the honeycomb). The vegetative (= living-active) form of the pathogen <em>P. larvae<\/em> occurs exclusively in the larvae of the honey bee. Each of these vegetative bacteria forms a spore, an extremely resistant form of survival. Up to 2.5 billion of these so-called endospores are formed per bee larva. Only the spores of the pathogen occur outside the larva. If the lavre ingests spores of <em>P. larvae<\/em>, e.g. via contaminated food, these germinate in the larva into a bacterium capable of reproduction and the cycle begins anew. Pathogen detection of spores<a href=\"#h-sporenanalyse-von-futterkranzproben\">(testing of feed crown samples<\/a>) is only possible through very complex laboratory diagnostics and not by means of a rapid test.<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-small-font-size\" id=\"h-\"><\/h3>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n<\/div>\n\n<div class=\"wp-block-advgb-accordions advgb-accordions-039575e7-3d5f-4f33-a1cb-be5cf1fa396a advgb-accordion-wrapper\" data-collapsed=\"true\">\n<div class=\"wp-block-advgb-accordion-item advgb-accordion-item\" style=\"margin-bottom:15px\"><div class=\"advgb-accordion-header\" style=\"background-color:#F2F2F2;color:#006CC2;border-style:solid;border-width:1px;border-color:#ffffff;border-radius:2px\"><span class=\"advgb-accordion-header-icon accordion-state\"><svg class=\"advgb-icon-closed\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M16.59,8.59L12,13.17L7.41,8.59L6,10l6,6l6-6L16.59,8.59z\"><\/path><\/svg><svg class=\"advgb-icon-opened\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M7.41,15.41L12,10.83l4.59,4.58L18,14l-6-6l-6,6L7.41,15.41z\"><\/path><\/svg><\/span><h3 class=\"advgb-accordion-header-title\" style=\"color:inherit\"><strong>scientific publication <\/strong>AFB rapid test for the detection and differentiation of&#8230; (English)<\/h3><\/div><div class=\"advgb-accordion-body\" style=\"border-style:solid !important;border-width:1px !important;border-color:#ffffff !important;border-top:none !important;border-radius:2px !important\">\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2026\/02\/Reinecke-et-al_Scientific-Reports-2026-16-2635.pdf\"><img loading=\"lazy\" decoding=\"async\" width=\"789\" height=\"1024\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2026\/02\/Publikation-AFB-Duplex-Schnelltest-789x1024.png\" alt=\"PDF: Immunoassays for the detection and differentiation of P. larvae, the agent of American foul brood (AFB) in honey bees\" class=\"wp-image-5094\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2026\/02\/Publikation-AFB-Duplex-Schnelltest-789x1024.png 789w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2026\/02\/Publikation-AFB-Duplex-Schnelltest-231x300.png 231w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2026\/02\/Publikation-AFB-Duplex-Schnelltest.png 1274w\" sizes=\"auto, (max-width: 789px) 100vw, 789px\" \/><\/a><\/figure>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><\/p>\n<\/div><\/div>\n<\/div>\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<div class=\"wp-block-advgb-accordions advgb-accordions-d4c1b9a7-0ef6-43ca-b530-9c01d12487a4 advgb-accordion-wrapper\" data-collapsed=\"true\">\n<div class=\"wp-block-advgb-accordion-item advgb-accordion-item\" style=\"margin-bottom:15px\"><div class=\"advgb-accordion-header\" style=\"background-color:#F2F2F2;color:#006CC2;border-style:solid;border-width:0px;border-color:#F2F2F2;border-radius:2px\"><span class=\"advgb-accordion-header-icon accordion-state\"><svg class=\"advgb-icon-closed\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M16.59,8.59L12,13.17L7.41,8.59L6,10l6,6l6-6L16.59,8.59z\"><\/path><\/svg><svg class=\"advgb-icon-opened\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M7.41,15.41L12,10.83l4.59,4.58L18,14l-6-6l-6,6L7.41,15.41z\"><\/path><\/svg><\/span><h3 class=\"advgb-accordion-header-title\" style=\"color:inherit\"><strong>Application Note<\/strong> Detection of American foulbrood by AFB duplex rapid test  <\/h3><\/div><div class=\"advgb-accordion-body\" style=\"border-style:solid !important;border-width:0px !important;border-color:#F2F2F2 !important;border-top:none !important;border-radius:2px !important\">\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:60px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-small-font-size\" id=\"h-application-note-duplex-schnelltest-amerikanische-faulbrut\"><strong>Application Note <\/strong>Duplex rapid test American foulbrood<\/h3>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><a href=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/AFB_Duplex-LFA_Application-Note_deu.pdf\"><img loading=\"lazy\" decoding=\"async\" width=\"727\" height=\"1024\" src=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut-727x1024.jpg\" alt=\"\" class=\"wp-image-4622\" style=\"width:261px;height:auto\" srcset=\"https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut-727x1024.jpg 727w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut-213x300.jpg 213w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut-768x1081.jpg 768w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut-1091x1536.jpg 1091w, https:\/\/www.fzmb.de\/wp-content\/uploads\/2025\/09\/Abb_PDF_Application_Note_Schnelltest_Amerikanische_Faulbrut.jpg 1230w\" sizes=\"auto, (max-width: 727px) 100vw, 727px\" \/><\/a><\/figure>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div><\/div>\n<\/div>\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<div class=\"wp-block-advgb-accordions advgb-accordions-b009b839-d577-46a7-bf37-5294fc7e0932 advgb-accordion-wrapper\" data-collapsed=\"true\">\n<div class=\"wp-block-advgb-accordion-item advgb-accordion-item\" style=\"margin-bottom:15px\"><div class=\"advgb-accordion-header\" style=\"background-color:#F2F2F2;color:#006CC2;border-style:solid;border-width:1px;border-color:#ffffff;border-radius:2px\"><span class=\"advgb-accordion-header-icon accordion-state\"><svg class=\"advgb-icon-closed\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M16.59,8.59L12,13.17L7.41,8.59L6,10l6,6l6-6L16.59,8.59z\"><\/path><\/svg><svg class=\"advgb-icon-opened\" fill=\"#006CC2\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"><path opacity=\"0.87\" fill=\"none\" d=\"M24,24H0L0,0l24,0V24z\"><\/path><path d=\"M7.41,15.41L12,10.83l4.59,4.58L18,14l-6-6l-6,6L7.41,15.41z\"><\/path><\/svg><\/span><h3 class=\"advgb-accordion-header-title\" style=\"color:inherit\"><strong>Contact<\/strong><\/h3><\/div><div class=\"advgb-accordion-body\" style=\"border-style:solid !important;border-width:1px !important;border-color:#ffffff !important;border-top:none !important;border-radius:2px !important\">\n<p><\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Contact<\/strong><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>fzmb GmbH<\/p>\n\n\n\n<p>Marie Mahnkopf<\/p>\n\n\n\n<p>Phone: +49 (0)361 &#8211; 663 9775<\/p>\n\n\n\n<p>EMail: <a href=\"mailto:mmahnkopf@fzmb.de\">mmahnkopf@fzmb.de<\/a><\/p>\n<\/div><\/div>\n<\/div>\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading\" id=\"h-hintergrundwissen\">Background knowledge<\/h2>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-der-erreger-der-amerikanischen-faulbrut\"><strong>The pathogen that causes American foulbrood<\/strong><\/h2>\n\n<p>The <a href=\"https:\/\/www.fli.de\/de\/aktuelles\/tierseuchengeschehen\/bienenkrankheiten\/bilder-amerikanische-faulbrut\/\">pathogen that causes American foulbrood (AFB)<\/a> is the spore-forming bacterium<em> <a href=\"https:\/\/de.wikipedia.org\/wiki\/Paenibacillus_larvae\">Paenibacillus larvae<\/a><\/em>. The highly infectious spores germinate exclusively in bee larvae but not in the adult animals. The germinated spores develop into bacteria, which multiply rapidly and lead to the death of the bee larvae. If an infection with the AFB pathogen is not detected in time, the deadly infection can quickly spread to neighboring bee colonies and also to wild honey bees. For this reason, American foulbrood (also known as malignant foulbrood) is not only a notifiable animal disease, but also requires immediate control measures.<\/p>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-eric-i-und-eric-ii-sind-verschiedene-erregertypen-der-afb\"><strong>ERIC I and ERIC II are different types of AFB pathogens<\/strong><\/h2>\n\n<p>There are different types of the American foulbrood pathogen. To be more precise, there are four different genotypes. Two of these genotypes are dangerous for bees and therefore clinically relevant. These genotypes of the spore-forming<em>Paenibacillus larvae <\/em>, which are lethal to bee larvae, are called   <em> <\/em><a href=\"https:\/\/www.fli.de\/de\/aktuelles\/tierseuchengeschehen\/bienenkrankheiten\/bilder-amerikanische-faulbrut\/\">ERIC I and ERIC II<\/a>. The two genotypes ERCI I and ERIC II occur worldwide and with the same frequency.<\/p>\n\n<p>Infection of the bee brood with one of the two genotypes leads to the death of the bee larvae. However, infection of a larva with ERIC II pathogens leads to a faster and stronger outbreak of the disease without the symptoms typical of ERIC I (e.g. stringy mass of rotten brood). This means that an ERIC II infection is either detected late or even remains undetected, thus facilitating the spread of the disease.<\/p>\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-bisherige-schnelltests-erkennen-nur-afb-erregervariante-eric-i\"><strong>Current rapid tests only detect AFB pathogen variant ERIC I<\/strong><\/h2>\n\n<p>According to studies by Benjamin <sup>Saville1<\/sup> and supported by current studies by the <sup>LIB2<\/sup>, the immunological rapid tests available on the market only detect the ERIC I genotype, but not ERIC II. However, as both genotypes occur to the same extent worldwide, an infection of bee larvae with the ERIC II pathogen of American foulbrood is often only recognized when the infection has already spread considerably.  <\/p>\n\n<p class=\"has-small-font-size\"><sup>1<\/sup> Saville, Benjamin Grayham. 2011. Differentiation of Virulent and Biological Control Paenibacillus larvae Strains Associated with American Foulbrood in Bee Hives. PhD thesis, University of York. <\/p>\n\n<p class=\"has-small-font-size\"><sup>2<\/sup> LIB: State Institute of Apiculture. Prof. Dr. E. Genersch <\/p>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-sicherer-afb-nachweis-beider-erregervarianten\"><strong>Reliable AFB detection of both pathogen variants<\/strong><\/h2>\n\n<p>The AFB duplex test for ERIC I + ERIC II enables quick and easy detection of a possible infection or exclusion of an infection of bee larvae by American foulbrood. However, it is necessary to test for <strong>both <\/strong>pathogen variants as genotype ERIC I and genotype ERIC II.   <\/p>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-warum-mussen-larven-getestet-werden-und-warum-keine-abstrichproben\"><strong>Why do larvae have to be tested<\/strong> (and why not swab samples)?<\/h2>\n\n<p>Unfortunately, it is not possible to detect the pathogen in American foulbrood from swab samples (e.g. from the comb). The vegetative (= living-active) form of the pathogen, <em>P. larvae<\/em>, is found exclusively in the larvae of honey bees. Each of these vegetative bacteria forms a spore within itself, an extremely resistant form of survival. Only these spores occur outside the larva. If the larva ingests spores of <em>P. larvae<\/em>, e.g. via contaminated food, these germinate in the larva to form a bacterium capable of reproduction and the cycle begins anew. Pathogen detection of spores is only possible through very complex laboratory diagnostics.     <\/p>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-sporenbildung-beim-erreger-der-amerikanischen-faulbrut\"><strong>Spore formation in the American foulbrood pathogen<\/strong><\/h2>\n\n<p>The bacterium <em>Paenibacillus larvae<\/em>is a spore-former. Spore formation enables bacteria to withstand extreme environmental conditions, for example. The active, vegetative bacterium forms a so-called <a href=\"https:\/\/de.wikipedia.org\/wiki\/Endospore\">endospore<\/a>. These spores can survive extreme conditions such as heat (up to 120 \u00b0C), drought and lack of nutrients, sometimes for decades. Once the environmental conditions are favorable again, the spore germinates into a new, active bacterial cell.    <\/p>\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<h2 class=\"wp-block-heading has-medium-font-size\" id=\"h-sporenanalyse-von-futterkranzproben\"><strong>Spore analysis of feed wreath samples<\/strong><\/h2>\n\n<p>For the detection of <em>Paenibacillus larvae<\/em>spores, it is currently recommended to test forage crown samples in special diagnostic laboratories. The bacteriological examination of feeder samples for the pathogen of American foulbrood (AFB) is very labor-intensive and takes approx. 1-2 weeks. <\/p>\n\n<p><br\/><br\/><br\/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>American foulbrood bee disease American foulbrood (AFB) is a highly infectious disease of bee larvae that leads to death. A quick and simple test of dead larvae on site provides immediate clarity as to whether an infection with American foulbrood is present. This offers the opportunity to initiate rapid countermeasures in the event of an [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":4159,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":"","footnotes":""},"class_list":["post-4190","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.1 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>AFB duplex rapid test for American foulbrood (AFB)<\/title>\n<meta name=\"description\" content=\"Simple and quick on-site detection of whether dead honey bee larvae are infected with AFB, even in the case of early\/atypical symptoms.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.fzmb.de\/en\/research\/detect-bee-infections-early\/tests-for-american-foulbrood\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"AFB duplex rapid test for the detection of American foulbrood bee disease\" \/>\n<meta property=\"og:description\" content=\"American foulbrood is a highly infectious disease of honey bee larvae. The AFB duplex rapid test with early detection provides quick and easy clarity as to whether dead larvae in the hive are infected with malignant foulbrood. 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