Research fields

BIOAFFINAS: Projects, where affine organic ligands (in particular antibodies) are created, modified, immobilized and used in technical applications (analysis, bio-processing technology), are summarized under this acronym ("bio-affine application systems").

Bio-affine ligands:
The institute operates a laboratory in which monoclonal antibodies and recombinant proteins for different applications (immunoassays, affinity chromatography, immuno-magnetic separation) are developed and produced. Moreover, the FZMB is involved in generating antibodies in vitro.
Modification and immobilization: In In cooperation with the FSU Jena (Prof. Heinze) at the FZMB was established a novel technique of coupling proteins via polysaccharides. It is the basis for the production of key components for technical applications (bio-chips, chromatography material and magnetic particle or color particle conjugates).
Biological analysis: At the FZMB, different binding assays in conventional (ELISA, test strips) and novel lab-on-a-chip formats (immunofiltration) are developed (see also POCT).
Bio-processing techniques: Since biological ligands have a high specificity, they are ideal tools for resource extraction from complex biological matrices. At the FZMB, a series of works are carried out to develop this potential for technically relevant processing processes (immunofiltration, monolithic chromatography, immunomagnetic separation).

Spectroscopy: At the FZMB, research works in the field of near-infrared spectroscopy (NIRS) and ion mobility spectroscopy (IMS) are primarily performed alongside UV/VIS and infrared spectroscopy (IR), which are established as routine methods. Strategic objective is the development of compact analytical instruments and innovative evaluation algorithms for applications in the field of veterinary medicine, food control and pharmaceutical process technology (in-line analysis of solids). A variety of spectrometer systems and evaluation procedures is accessible, in particular the creation of chemometric models based on multivariate statistical techniques (PCA, PLS) and of neural networks. Since the appropriate reference methods are well established and accredited in the departments of food laboratory, biotechnology and veterinary medicine, a holistic approach can be pursued and application-oriented complete solutions developed.

KJM = Cartilage, Joints, movementStarting point of the work is the veterinary medicine, more precisely joint diseases in the horse. The strategic objective is to provide novel diagnostic methods for the evaluation of degenerative processes in the joint and new therapeutic approaches for cartilage regeneration. The thereby developed approaches are not limited to the veterinary medicine and initiate a series of cooperation projects with focus on human medicine.

Cartilage diagnostics: Based on classic gait analysis in the horse, new methods were and will be developed to objectify cartilage damages using NIR spectroscopy (endoscopy) and immunoassays (e.g. matrix metalloproteinase 9).
Cartilage regeneration: At the Institute a method to create three-dimensional scaffold-free cartilage constructs and a new surgical method based on paste-like cartilage implants have been established successfully in an animal model.
Cartilage model: Based on the work on scaffold-free cartilage constructs, a novel in vitro test method for use in clinical trials has been established.

“Point-of-Care-Testing (POCT)” is a modern trend in medical diagnostics, which aim is to provide a fast on-site analysis based on small portable analyzers to avoid logistical and technical problems raised by centralized diagnostics. The Institute develops such decentralized testing concepts as well as appropriate equipment and test kits for use in veterinary medicine and human medicine. In addition, these technologies are also used to provide innovative solutions for food control and in-process control in pharmaceutical and biotechnological processes.

Immunochromatography: At the Institute, a series of rapid tests for the detection of inflammation markers, cardiac markers, toxins and infections (viruses, microorganisms) as well as in the classic test strip format ("lateral flow") were established in the micro-column format (ABICAP). Thus, synergies with BIOAFFINAS can be used efficiently to develop product innovations for and with industry partners.
Lab-on-a-chip: Micro-structured analysis chips are used for the realization of complex analysis processes in particular in POCT-situations for several years. They represent a further development of the rapid tests according to 4.1. The FZMB is dealing with appropriate technological solutions.
IMS: With the ion mobility spectroscopy volatile substances are detected quickly and efficiently using small portable devices. The FZMB develops applications in the fields of veterinary medicine (respiratory gas analysis) and food analysis.
NIR: A portable spectrometer for industrial use was developed at the Institute. In addition to traditional applications in the meat industry (determination of fat, water protein, BEFFE) more applications in the field of process control (yeast, granulation) are addressed.
FI2FE: Fluorescenc Imaging after Immunofiltration Enrichment is an innovative fast method for the detection of cells. It is particularly suitable when a few cells have to be detected in large volumes.