Services

We offer various molecular biological, microbiological and biotechnological testing and development services. This can be studies or the implementation of a project. We accompany you from the idea to the market launch of a new product or a new process.

Our accredited food laboratory at the fzmb also offers a wide range of services in the field of microbiological and chemical analyses.

The veterinary clinic at our institute in Bad Langensalza provides care for small and large animals in almost all medical fields.

Diagnostic systems

Microarray development
Development of multiparameter tests
High precision dispensing (Life Sciences)
Development of immunoassays (LFA/ ELISA)
Coupling of proteins to tracer molecules

Development and production of microarrays

Microarrays consist of a large number of microscopic spots that can be precisely deposited on a solid surface by high-precision dispensers. For example, more than 700 spots can be applied in a single well of a 96-well microtiter plate.

 

Customized development

In the business unit INTER-ARRAY at the fzmb we accompany you through all development phases of microarrays from feasibility studies to pilot production. After successful development, we are available to you as a contract manufacturing partner with our ISO-certified production capacities.

 

Microarray quality control

The focus is on the high quality of the microarray products. For production, the dispensing systems operate in a climate-controlled environment with inline QC software, ensuring quality control of all arrays produced.

 

 

Information

For more information, visit our website at www.inter-array.com.

 

 

Contact

Katrin Frankenfeld

Head of Biotechnology Department

phone +49 (0)360 – 833 141

Email

 

 

 

 

Development and production of multiparameter tests

Protein and antibody microarray technology offers enormous potential for a wide range of diagnostic applications. Miniaturized, parallelized immunoassays as well as DNA-based microarrays are ideal for generating maximum diagnostically relevant information with small sample and reagent volumes. A microarray thus saves a lot of time, valuable samples and expensive raw material.

Application in human and veterinary medicine

The use of microarrays in human and veterinary diagnostics is of interest for all applications where multiple analytes can be determined from a single sample.

Typical examples of multiparameter analysis are allergy and food intolerance tests, the diagnosis of autoimmune, metabolic and infectious diseases, and cancer diagnostics. The technology offers excellent prospects for new ways of individualized diagnostics and personalized medicine.

Multi-parameter analysis in different formats

In the business area INTER-ARRAY at the fzmb we carry out new developments of various multiparameter tests. Standard detection formats such as ELISA, lateral flow strips, and microfluidic assays can be used, as well as custom platforms.

Our team offers flexible support for feasibility, validation as well as production projects for various microarray based assays. In addition to completely new development, the transfer of existing tests to the microarray platform is also possible.

Information

For more information, visit our website at www.inter-array.com.

Contact

Katrin Frankenfeld

Head of Biotechnology Department

phone +49 (0)360 – 833 141

Email

High precision dispensing

In order to apply very small volumes of capture molecules (from picoliters to microliters) to any surface with high precision, a lot of experience is required in addition to very good dispensing systems. Our business unit  INTER-ARRAY at fzmb is equipped with a variety of state-of-the-art microdispensers and has many years of professional know-how.

From design to data analysis: Exclusive printing offers

With our established technologies for high-precision dispensing and our years of experience from design to data analysis, we create an exclusive printing offering in the life science sector. We offer this all-round service at an attractive price/performance ratio. The focus is on high quality and short production times.

Spotting of various substances

Substances that can be spotted include antibodies, other proteins and peptides, oligonucleotides, carbohydrates, cells and cell lysates, particles and chemical compounds, and special customer-supplied molecules. Substrates to be coated include glass and polymer slides, microtiter plates and microcavities, membranes and microfluidic chips, wafers, biosensors, and customized surfaces.

Information

For more information, visit our website at www.inter-array.com.

Contact

Katrin Frankenfeld

Head of Biotechnology Department

phone +49 (0)360 – 833 141

Email

Immunoassay development

The lateral flow assay (LFA) is an immunological strip test. It combines the principle of thin-layer chromatography with antibody-based detection (colorimetric/fluorescence). An LFA is  the basis for cost-effective, highly sensitive and reliable detection of a wide range of analytes.

As a point-of-care test (POCT), lateral flow assays find multiple applications e.g. in immunodiagnostics or in the analysis of environmental and food samples.

The ELISA (Enzyme-linked Immunosorbent Assay) is used for the qualitative and/or quantitative detection of an analyte (antigen) in a sample. In addition, many samples can be analyzed in parallel and automated in a laboratory environment.

We develop LFAs and ELISAs from feasibility to small-scale production or preparation for large-scale production. These specific immunoassays can be used to diagnostically detect a wide variety of analytes in complex sample matrices. Highly specific and affine monoclonal and polyclonal antibodies are required for reliable selective binding of the analyte in a liquid phase or tissue sample. Their production and application-related characterization is one of our core competencies.

Reliable detection of biological structures with high sensitivity

Imunoassays can detect the analytes of interest down to the nanogram range, in some cases even the picogram range. Thereby,  qualitative and quantitative detections from a wide variety of biological samples with complex compositions are possible without pretreatment.

At fzmb, rapid highly specific test systems with colorimetric, fluorescence or chemiluminescence-based readout for any parameter are developed.

Performance overview

  • Development of ELISAs for the qualitative detection of micro- and macromolecules (formats: indirect ELISA, sandwich ELISA).
  • Development of ELISAs for qualitative detection of analytes (format: sandwich ELISA).
  • Development of lateral flow assays (LFA) for qualitative detection of biomarkers.
  • Optimization of assay components and steps for the benefit of sensitivity and assay speed
  • Validation of the developed ELISA/ LFA

 

Contact

Andrea Helming

phone +49 (0)361 – 663 97 75

Email 

Content follows

Specific antibodies

Production of monoclonal antibodies
Production of recombinant antibodies (horse)
Production of polyclonal antibodies
Application-related matched pair antibodies

Production of monoclonal antibodies

Monoclonal antibodies can be generated for almost any antigen or epitope. From this, diagnostic test systems of various formats can be established for the detection of these structures, as they bind the desired target structure in a highly specific manner.

Rapid development and characterization for the specific application

We develop specific monoclonal antibodies in the host organism mouse. In addition to antibody production, we can also supply the equivalent antigen (recombinant and native proteins, peptides, microorganisms and their structures). For rapid development downstream, we offer antibody characterization and matched pair analysis. We use the classic hybridoma technique. The antibodies can thus find application e.g. in high-quality and effective test systems for the specific diagnosis of the desired antigens.

 

Performance overview

  • Production/procurement of the antigen (immunization, screening)
  • Immunization of laboratory animals
  • Titration of the sera (verification of the immune response)
  • Fusion of mouse spleen cells with mouse myeloma cells (hybridoma technique).
  • Screening for antibody-secreting clones, specificity testing
  • Characterization of antibodies (isotyping, expression performance, affinity to the antigen of interest).
  • Establishment of a permanent cell line for the selected clones, incl. Cryopreservation and storage in liquid nitrogen
  • Antibody production (cell culture supernatant) and isolation of antibodies (affinity chromatography)
  • Integration in immunoassays of different formats (ELISA, LFA, IFAT)

 

 

Contact

Andrea Helming

phone +49 (0)361 – 663 97 75

Email

 

Overview of antibody-producing cell lines at the fzmb

 

Antibodies directed against

  • American foulbrood
  • Aminocellulose
  • Anti-Trypsin (Alpha1Pi)
  • Archaea (e.g. Methanosarcinae)
  • BBI
  • Clostridium botulinum toxin C
  • Clostridium botulinum toxin A
  • Burkholderia mallei
  • Brucella abortus
  • Complement component C3A
  • Coxiella burnetii
  • Deoxynivalenol
  • DY dyes and quenchers
  • Factor X
  • Factor Xa
  • Heparin Binding Protein
  • HIS Day
  • Human Immunoglobulin A
  • Human Immunoglobulin G
  • Lactobacillus brevis
  • Lactoferrin
  • Legionella pneumophila
  • Lipid A (Salmonella)
  • Listeria monocytogenes
  • LukF
  • Lunasin
  • MAP, Mycobacterium avium paratuberculosis (inactivated cells).
  • eMMP9 (equine matrix metallo proteinase 9)
  • NDV, Newcastle Disease Virus
  • fNTproBNP (feline natriuretic brain peptide)
  • cNTproBNP (canine Natriuretic Brain Peptide)
  • Parvovirus
  • PBP2a
  • Pediococcus damnosus
  • Pediococcus inopinatus
  • Pneumocystis jirovecii
  • Procalcitonin
  • Rotavirus
  • SAG1 (Toxoplasma gondii)
  • Salmonella
  • SARS-CoV-2 (human Severe Acute Respiratory Syndrome Coronavirus 2)
  • SP-10 (human sperm protein, ACRV-1)
  • Transferrin
  • Troponin
  • Felines trypsinogen
  • Zearalenone

 

Contact

Andrea Helming

phone +49 (0)361 – 663 97 75

Email

Production of recombinant antibodies (horse)

In human medicine, so-called chimeric (about 75% human) and humanized antibodies have been used for more than 20 years, and there are now a large number of approved therapeutic antibodies in humans. As a company with strong ties to veterinary medicine, our mission is to develop novel immunological therapies for companion animals.

Specific antibodies for therapy

We are developing recombinant equine-specific antibodies for subsequent therapeutic use in horses. The current focus is the development of an antibody against matrix metalloproteinase 9 (MMP-9). This antibody is intended to serve as a specific therapeutic agent, for example, for the targeted treatment of arthritis, wound healing disorders and equine sarcoid. But also in other diseases in which an overexpression of matrix metalloproteinase 9 (MMP-9) is causally involved, a new therapeutic option is to be created. In this way, pathological processes that arise due to the tissue-degrading properties of MMP-9 can be specifically stopped at the site of their development.

 

recombinant equine immunoglobulins

Molecular genetic methods have been developed for the production of equine recombinant antibodies in cell culture. Furthermore, these techniques can be used to produce other equine immunoglobulins, for example, as passive immunotherapeutics against equine infectious diseases. Based on the choice of subtype specificity and the regulatory Fc region, beyond the desired antigen specificity, the potential as a modulator can be used to strengthen the body’s immune system, e.g. against an existing cancer.

Go with us conversation about your concern and issue. Our ambition is to work with you to lay the foundations for innovative and specific immunotherapy for the horse.

If we have aroused your interest, please feel free to contact us.

Contact

Dr. Ina-Garbriele Richter

phone +49 (0)360 – 833 177

Email

Production of polyclonal antibodies

We produce artificial human immune sera by means of in vitro immunization.

 

Contact

Dr. Ina-Gariele Richter

phone +49(0)360 – 833 177

Email

Matched Pair Antibodies

Whether a chosen matched-pair antibody works well in a planned application depends on many factors and cannot be predicted.

We therefore not only offer the development of specific monoclonal antibodies (including the identification of matched pairs) for e.g. Immunoassay developments on.

For best performance of a Matched Antibody Pair, we test the performance of these antibody pairs in the desired application. This early testing of antibodies in the target application (ELISA, LFA with immunogold or fluorescent nanoparticles, IFAT) enables targeted antibody selection or development and thus allows the best possible performance.

 

Performance overview

  • Development of monoclonal antibodies against desired target structures
  • Antibody characterization (isotyping, expression)
  • Matched pair analysis in target format (sandwich ELISA, lateral flow assay LFA):
    • Coupling to structures for colorimetric or fluorimetric detection (biotin, HRP, AP, fluorescent dyes).
    • Immobilization of the catcher candidates
    • Detectors analysis
    • Testing of possible combinations
  • Buffer optimization

 

Contact

Andrea Helming

phone +49 (0)361 – 663 97 75

Email:

 

 

Biological recyclables

Virus-like particles (VLP) for test development
Inactivation of microorganisms
BioCellulose (Made in Germany)
image of Virus like particle, polyomavirus

Virus-like particles (VLP) of human polyomavirus JCV as purified antigen for test development.

The human polyomavirus JCV-derived virus-like particles (VLPs) consist of the major structural protein VP1. Five VP1 proteins each assemble into a VP1 pentamer (capsomer). 72 of these capsomers form an approximately 45 nm virus-like particle (VLP). VLP is produced recombinantly in insect cells in cell culture.

VLP-based assays are essential for determining prior exposure to JCV in epidemiologic studies. Similarly, they find use for serologic diagnosis in patients at risk for polyomavirus-associated disease or showing early signs of complications due to polyomavirus reactivation.

If you need recombinantly expressed JCV-VP1-VLP for the development of your own assays or if you are interested in a joint development, we are looking forward to your contact.

 

 

Contact

Dr. Ina-Gabriele Richter

phone +49(0)360 – 833 177

Email

 

Inactivated bacteria

The use of inactivated bacteria as control material or for immunization for antibody production can minimize the risk of infection and contamination. For this purpose, bacteria are conventionally inactivated by thermal or chemical treatment. This material is unsuitable for many applications because the morphology and antigenicity of the bacteria are altered.

 

UV inactivation of microorganisms

UV-inactivated microorganisms, on the other hand, are particularly well suited as microbiological con-trol material for scientific or medical purposes. The high-energy UV light damages the DNA and thus causes the inactivation of the bacteria. The macro-molecular outer surface is well preserved. Thus, these bacteria are particularly well suited for generating antibodies against surface antigens. If inactivated bacteria are used as reference material to vital bacteria, comparable results are obtained.

 

Customized inactivation of microorganisms

We offer microorganisms as whole cell preparations (inactivated bacteria such as Legionella, Streptococcus, etc.) as standardized, independent quality controls for the evaluation of molecular tests. In addition, we can perform customized inactivation of bacteria (UV inactivation, heat inactivation, formalin inactivation) according to your specifications. You determine the desired concentrations, buffers and additives.

Please feel free to contact us so that we can discuss your individual request.

 

Contact

Dr. Ina-Garbriele Richter

phone +49 (0)360 – 833 177

Email

 

 

 

BioCellulose (Made in Germany)

 

Ultra thin & ultra light

Natural skin feeling

Our BioCellulose is a pure natural material. It is significantly purer than plant cellulose and consists of a stable mesh of ultra-thin fibers that adapt perfectly to any skin contour. The masks are particularly light and transparent and therefore interesting even for those who have not used fleece masks so far.

BioCellulose as active ingredient carrier

In its meshwork of millions of fine fibers, our BioCellulose stores cosmetic active ingredient solutions for the treatment and care of the skin. Due to the optimal adaptation to the skin surface, the active ingredient molecules are specifically delivered to the skin and very good cosmetic results are achieved.

Application

Easy application in the beauty salon or at home

The BioCellulose masks are incredibly light and thin. As a result, they lie almost invisibly on the face. The application of the masks is very simple. The carrier material used allows easy application of the BioCellulose to the skin. The two mask parts allow adjustment to any face size. Due to the extremely good adhesion of the BioCellulose to the surface of the face, the client has a high degree of freedom of movement during the treatment.

Ideal combination with apparative treatment methods

BioCellulose masks provide a unique basis for combination with apparative treatment methods. The material of the masks allows, in addition to an optimal release of active ingredients, a perfect transfer of the physical effects to positively influence regeneration processes of the skin and stimulate metabolic processes.

Production

Development, production and packaging

We act as a contract manufacturer for the production of cosmetic masks according to customer requirements. Our BioCellulose is biotechnologically extracted from glucose in Germany and processed into masks. We impregnate the masks with our customers’ active ingredients or provide a product formulation. We are happy to advise on the choice of active ingredient.

Research and development

We implement the ideas that our customers bring to us. The production of samples is part of our service offer. With our own perfectly equipped laboratories, our competent chemists, biologists and laboratory technicians are able to offer comprehensive research and development services.

Quality

We have been producing BioCellulose at the highest level for 20 years. Our experienced team works to produce high quality products to exacting standards. The GMP-compliant production is certified according to DIN ISO 9001. The product is dermatologically tested and is manufactured entirely in Germany. We guarantee fast order processing.

 

Contact

Dr. Ina-Gabriele Richter

phone +49(0)360 – 833 177

Email

 

Analysis of food, drinking water and cosmetics

Food laboratory – general information
Microbiological and chemical analysis of food
Microbiological testing of cosmetics
Examination of air, objects, environmental samples
Drinking water testing
other signatures

Food laboratory – general information

The safety of our food has become an increasing focus for consumers in recent years. Food scandals are increasingly dominating the headlines in the public media.

Do you want to offer your customers safe, high-quality food or even avoid recalls?

Our competent and experienced team supports you from consulting to analysis.

Please note: When testing as an official self-control test (submission to authorities), sampling by the laboratory is mandatory. Our accredited internal and external samplers are at your disposal for this purpose.

We not only carry out microbiological and chemical tests within the framework of our own inspections, but also provide support, e.g. in questions of food law, the preparation and testing of labels or practical problems in the production process.

Our field staff will be happy to advise you on site by individual arrangement and will also collect samples.

 

Contact

Steffi Leuschke

State-certified food chemist

phone +49 (0)360 – 833 173

Email

 

 

Microbiological and chemical analysis of food

We perform microbiological, chemical and chemical-physical examination of food, product specific among others on:

  • Pathogen
  • Spoilage agent
  • Compliance with legal requirements (e.g. VO 2073/2005, Zusatzstoff-Zulassungsverordnung, Käseverordnung)
  • Compliance with microbiological guideline and warning values of the German Society for Hygiene and Microbiology (DGHM)
  • aw value and pH value
  • Determination of food ingredients
  • Compliance with specifications of the guiding principles of the German Food Book
  • Nutritional value determination “BIG 7
  • Determination of ochratoxin A in cereals and cereal products by HPLC.
  • Detection of antibodies against Salmonella serovars of groups B, C, D and E in meat juice and blood products by ELISA, entry of results in the Qualiproof database.
  • Checking of markings for legal conformity

 

Contact

Steffi Leuschke

State-certified food chemist

phone +49 (0)360 – 833 173

Email

Microbiological testing of cosmetics

Microbiological product safety is one of the most important factors in the safety evaluation of cosmetic products. Our analytical laboratory tests the microbiological quality of cosmetic products as well as the raw materials used. Within the scope of operational hygiene monitoring, we offer manufacturers of cosmetics various tests of process water or production water, of room air and surfaces as well as of packaging materials.

The spectrum of our laboratory includes the following analytical methods:

  • Preservation load test

(Evaluation of the preservation of cosmetic formu-.

ren)

  • Testing for microbial contamination
  • Detection of specific microorganisms
  • Checking the cleaning and disinfection of surfaces and consumer goods
  • Checking the indoor air quality

 

Contact

Steffi Leuschke

State-certified food chemist

phone +49 (0)360 – 833 173

Email

Examination of air, objects, environmental samples

  • Checking the cleaning and disinfection of surfaces and consumer goods
  • Checking the indoor air quality
  • Verification of personnel hygiene

 

 

Contact

Steffi Leuschke

State-certified food chemist

phone +49 (0)360 – 833 173

Email

Drinking water testing

The testing in our laboratories of drinking water is carried out in accordance with the Ordinance on the Quality of Water for Human Consumption (Drinking Water Ordinance – TrinkwV 2001):

  • Checking the microbiological quality of drinking water (colony count at 22°C and 36°C, coliforms, Escherichia coli, enterococci)
  • Determination of Pseudomonas aeruginosa
  • Determination of Clostridium perfringens
  • Testing of drinking water installations for Legionella spp.

 

Contact

Steffi Leuschke

State-certified food chemist

phone +49 (0)360 – 833 173

Email

Content follows

Data Science & Optical Analytics

Chemometric data analysis for spectroscopy and spectral imaging
Determination of optical material properties and simulation of optical setups
Feasibility studies and validations of optoelectronic sensors
Rapid Prototyping / 3D Printing

Example: Chemometric data analysis in pharmaceutical production

In the pharmaceutical production of tablet granules, it is important that active ingredients are homogeneously distributed. By using special camera systems, we can determine a pinpoint concentration determination in real time.

The evaluation of spectral information for the quantification of quality-determining properties of a sample is model-based. The chemometric calibration model is based on spectral measurement data and reference parameters of a large number of previously measured samples.

Using AI-based mathematical methods, we develop chemometric models to calculate sample properties (e.g., chemical composition) from spectral information.

 

Do you have a question?

We support you in the selection and configuration of spectral sensor technology, in the planning and execution of measurement series for calibration. Furthermore, we develop AI-based software components for the model-based evaluation of your measurement data.

 

Overview services

    • Development of chemometric regression and classification models
    • Development of new AI-based methods
    • Optimization of existing chemometric models
    • Model validations
    • Reference analytics

 

Contact

Florian Meuche

Department Manager Equipment Development

phone +49 (0)360 – 833 194

Email

Determination of optical material properties

Optical material properties are assessed by measuring absorption, reflection, scattering and fluorescence. Our highly specialized optics laboratory has setups and measurement equipment for measuring the absorption, reflection and scattering of materials in the wavelength range from UV to MIR. Furthermore, we offer fluorescence spectroscopic measurements.

 

Simulation of optical setups

For finding a suitable light source (point-shaped, rod-shaped, spherical, planar) for illuminating a region-of-interest (ROI = point, area, body) we can perform simulations of optical setups.

 

Contact

Florian Meuche

Department Manager Equipment Development

phone +49 (0)360 – 833 194

Email

Analysis of ingredients and materials

Do you want to assess the quality of your food (beverages, long-life bakery products, flour, spices, herbs, cereals, coffee and tea)? Or you would like to professionalize your material analysis (pharmaceuticals, cosmetics, building materials, textile analysis, sewage residues, waste water)?

The technical solution developed by us for your individual problem will enrich your process practice. The use of optical sensors to solve practically relevant metrological problems requires an optimized configuration of the measurement environment as well as the sensor parameters.

Our experts support you with the configuration and optimization of your measurement setups and prototypes with the help of our highly specialized spectroscopy laboratory. We perform measurement series to characterize the metrological performance data of your sensor technology and find intelligent solutions to convert your sensor application into a marketable product.

Performance overview

    • Application-specific verification of the suitability of optical measurement methods
    • Metrological characterization of optoelectronic sensors
    • Planning, execution and evaluation of test series

 

Contact

Florian Meuche

Department Manager Equipment Development

phone +49 (0)360 – 833 194

Email

You plan the design of a mechanical component, e.g. for the assembly of a device prototype. To this end, we offer the creation of CAD models and the rapid production of prototypes using 3D printing.

    • CAD modeling and rapid prototyping

 

Contact

Florian Meuche

Department Manager Equipment Development

phone +49 (0)360 – 833 194

Email